NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins.
These are typically small secreted proteins characterized by their ability to bind small, hydrophobic molecules in a structurally conserved pocket formed by Beta-pleated sheet, to bind to specific cell-surface receptors and to form macromolecular complexes.
NGAL has many synonyms: it also known as NL (neutrophil lipocalin; HNL: rat NL), lipocalin, oncogene protein 24p3 or uterocalin (in the mouse) and neu-related lipocalin or 25kD alpha 2-microglobulin-related protein6 (in the rat).
Rat NGAL consists of a single disulfide-bridged polypeptide chain of 178aa residues with a calculated molecular mass of 22kD, but glycosylation increases its apparent molecular mass to 25kD.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine NGAL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Thr23-Gln199 of porcine NGAL, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
