Nuclear transcription factor Y subunit gamma is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes
This gene product, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association.
The resulting trimer binds to DNA with high specificity and affinity.
Subunits B and C each contain a histone-like motif.
Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.
Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NFYC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val263 of human NFYC, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
