(1997) cloned and characterized 3 splice variants of NFIB.
NFIB1 and NFIB2 are translated from an 8.7-kb message that encodes proteins containing an N-terminal DNA-binding domain and a C-terminal transactivation domain.
NFIB3 is a truncated 188aa protein, translated from a 1.8-kb message, that contains only the N-terminal DNA-binding domain.
Northern blot analysis detected the 1.8-kb NFIB3 message in HeLa cells and fibroblasts, as well as in several human tissue samples.
Coexpression of NFIB3 with active isoforms of NFIB, NFIC, and NFIX, however, led to a strong repression of transcription by the other NFI proteins.
Gel shift analysis indicated that NFIB3 formed heterodimers with the other NFI proteins and reduced their ability to bind DNA.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NFIB.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Gln233 of human NFIB, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
