NFATC2 is a member of the nuclear factor of activated T cells (NFAT) family.
The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR).
This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex.
This complex plays a central role in inducing gene transcription during the immune response.
Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
A truncated form of NFATP, expressed as a recombinant protein in bacteria, bound specifically to the NFAT site of the murine IL2 promoter and formed a transcriptionally active complex with recombinant FOS and JUN.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NFATC2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala392-Ala574 of human NFATC2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
