SYNE2 is a nuclear outer membrane protein that binds cytoplasmic F-actin.
This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus.
Several transcript variants encoding different isoforms have been found for this gene.
SYNE2 contains multiple spectrin repeats and a 60aa C-terminal region homologous to the Drosophila protein Klarsicht.
RT-PCR analysis showed low to moderate expression of 2 SYNE2 splice variants in most tissues examined, including skeletal and cardiac muscle, kidney, liver, pancreas, and placenta, and indicated that alternative splicing patterns are conserved between SYNE1 and SYNE2.Nesprin-2-gamma also has a bipartite nuclear localization signal and a leucine zipper motif.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Nesp2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu6-Gln296 of human Nesp2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
