Nestin is a type VI intermediate filament (IF) protein.
These intermediate filament proteins are expressed mostly in nerve cells where they are implicated in the radial growth of the axon.
Seven genes encode for the heavy (NF-H), medium (NF-M) and light neurofilament (NF-L) proteins, nestin and alpha-internexin in nerve cells, synemin alpha and desmuslin/synemin beta (two alternative transcripts of the DMN gene) in muscle cells, and syncoilin (also in muscle cells).
Members of this group mostly preferentially coassemble as heteropolymers in tissues.
Steinert et al.
has shown that nestin forms homodimers and homotetramers but does not form IF by itself in vitro.
In mixtures, nestin preferentially co-assembles with purified vimentin or the type IV IF protein -internexin to form heterodimer coiled-coil molecules.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat NES.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg197-Asn462 of rat NES, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
