Neudesin was expressed in early development in mouse embryonic cerebral cortex in neural precursor cells.
Studies in culture showed that neudesin promoted neuronal differentiation and inhibited differentiation of astrocytes.neudesin has a predicted cytochrome b5-like heme/steroid-binding domain, and determined that Fe(III)-protoporphyrin IX is required for its neurotrophic activity.
An anti-neudesin antibody detected a 15.6kD band in media from cultured Neuro2a cells and was used in experiments to confirm that the protein binds heme.
In mice, in situ hybridization showed abundant expression of neudesin in developing brain and spinal cord, and RT-PCR of postnatal tissues demonstrated expression in brain, heart, lung, and kidney.
The protein was expressed in neurons but not in glial cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NENF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly32-Phe172 of human NENF, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
