NELL2 encodes a cytoplasmic protein that contains epidermal growth factor (EGF) -like repeats.
The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation.
A similar protein in rodents is involved in craniosynostosis.
An alternative splice variant has been described but its full length sequence has not been determined
NELL2 open reading frame encodes an 816aa polypeptide.
The amino acid sequence of NELL2 showed 80% identity to that of chicken 'nel,' and Watanabe et al.
(1996) stated that NELL2 may be the actual interspecies homolog of the 'nel' gene.
NELL2 was strongly expressed as a 3.6-kb transcript in human adult and fetal brain tissues
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NELL2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro64-Lys331 of human NELL2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
