NEK2 has an N-terminal catalytic domain and a very basic C-terminal region.
Overall, NEK2 shares 36 to 38% sequence identity with nimA, Nek1, and human NEK3.
Using Western blots of extracts of synchronized HeLa cells, the authors found that NEK2 protein was almost undetectable during G1, but accumulated progressively throughout S phase, reaching maximal levels in late G2
NEK2B encodes a deduced 384aa protein with a calculated molecular mass of 44.9kD.
It diverges from the longer isoform, NEK2A, following 370aa and lacks a number of important regulatory motifs in the extreme C terminus of NEK2A.
The 2 isoforms exhibited distinct patterns of cell cycle-dependent expression in U2OS cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NEK2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys125-Asn393 of human NEK2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
