84-4429-37　NEIL3 (Nei Endonuclease VIII Like Protein 3, FPG2, hFPG2, hNEI3, Endonuclease 8-like 3,DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3) 200ul　364329

特徴

• NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family.
• These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction
• The deduced 605aa protein has a calculated molecular mass of 67.9kD.
• Unlike NEIL1, NEIL3 contains a RAN-binding protein-like zinc finger motif and C-terminal zinc ribbon domains.
• Northern blot analysis of several human tissues detected a 2.4-kb transcript only in testis and thymus.
• Fluorescence-tagged NEIL3 colocalized with RPA2 in the nuclei of transfected HeLa cells and was excluded from nucleoli.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.