84-4429-36　NEIL1 (Nei Endonuclease VIII Like Protein 1, NEI1, hFPG1, FPG1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Nei-like protein 1, DNA glycosylase/AP lyase Neil1) 200ul　364328

特徴

• NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family.
• These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction
• Bacterial Fpg/Nei proteins contain 2 structural domains: an N-terminal 2-layered beta sandwich and a C-terminal 4-helix bundle, which includes a helix-2-turns-helix (H2TH) motif and a zinc finger.
• The deduced 390aa human NEIL1 protein has conserved residues in 6 highly conserved regions that span both structural domains of the bacterial Fpg/Nei proteins, but it lacks the characteristic zinc finger motif.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.