NDUFS1 belongs to the complex I 75kD subunit family.
Mammalian complex I is composed of 45 different subunits.
It locates at the mitochondrial inner membrane.
This protein has NADH dehydrogenase activity and oxidoreductase activity.
It transfers electrons from NADH to the respiratory chain.
This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme.
It may form part of the active site crevice where NADH is oxidized.
Mutations in this gene are associated with complex I deficiency.
The predicted 727aa human 75kD Fe-S protein contains a 23aa presequence and is 97% identical to the bovine homolog.
Various cysteine-rich motifs similar to those found in rubredoxins, the Reiske Fe-S protein in Neurospora, and 4 Fe-4S ferredoxins are present in the protein sequence.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NDUFS1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala524-Cys727 of human NDUFS1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
