LY95 is a type I transmembrane protein with a 21aa leader sequence, a 169aa extracellular region, a 23aa transmembrane protein, and a 63aa cytoplasmic tail.
The potential N- and O-linked glycosylation sites are apparently occupied since the observed molecular mass of LY95 is 44kD as opposed to the predicted 24kD
The cytoplasmic tail contains the amino acid sequence ILYHTV, which fits the consensus sequence for an immunoreceptor tyrosine-based inhibitory motif (ITIM).
Northern blot analysis revealed that expression of 3.7- and 1.2-kb LY95 transcripts are restricted to interleukin-2 (IL2)-activated NK cell lines and clones.
IL2-activated NK cells have enhanced activity in vitro and in vivo.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NCR2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln22-Ser120 of human NCR2 expressed in E. coli.
