Natural cytotoxicity triggering receptor 1 is a protein encoded by the NCR1 gene.
By screening a human NK cell cDNA library, Pessino et al.
(1998) cloned the cDNA for NKp46, or LY94, an Ig superfamily member that may cooperate with other activating receptors.
The LY94 cDNA encodes a 304aa type I transmembrane protein.
The extracellular region is preceded by a 21-residue signal peptide with 2 cysteine-bridged C2-type Ig-like domains.
A stem connects the extracellular domain to the apparently short transmembrane domain of 19 aa, which includes an arginine.
The intracellular region contains 30aa and is rich in basic residues, but does not contain any immunoreceptor tyrosine-based activating motifs (ITAM).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NCR1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln17-Thr211 of mouse NCR1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
