NCOA3 is a transcriptional coactivator protein which contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity.
NCOA3 is recruited to DNA promotion sites by ligand activated nuclear receptors.
NCOA3 in turn acylates histones which makes downsteam DNA more accessible to transcription.
Hence NCOA3 assists nuclear receptors in the upregulation of gene expression
This variant, which they called AIB-delta-3, encodes a 130kD protein lacking the N-terminal basic helix-loop-helix domain and the first of the 2 PAS dimerization domains of full-length AIB1.
Northern and Western blot analyses detected higher AIB-delta-3 expression in breast tumor samples and cell lines than in normal breast tissue.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NCOA3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly1023-Ala1304 of human NCOA3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
