Neutrophil cytosol factor 2 is the 67kD cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.
This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome.
Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease
Using neutrophils from a patient with p67-deficient CGD, Okamura et al.
(1990) confirmed that the p67 protein functions in the respiratory burst mediated by NADPH oxidase and that p67 may be complexed to p47-phox (NCF1) while it participates in oxidase activation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NCF2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Lys354-Val525 of mouse NCF2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
