(1997) performed exon trapping from chromosome 21-specific cosmids.
They identified a novel trapped exon that showed homology to NCAM1.
They cloned the corresponding cDNA from a human fetal brain cDNA library and reported that it encodes an 837aa polypeptide containing 5 immunoglobulin-like domains, 2 fibronectin type III domains, and 1 predicted transmembrane domain.
The overall size and structure of the predicted protein are similar to the shorter isoform of NCAM1.
The authors were unable to identify any longer cDNAs.
Paoloni-Giacobino et al.
(1997) showed by Northern blot analysis that the NCAM2 gene is expressed as mRNAs of 9.5 and 4.4 kb in fetal and adult brain; they observed these and other transcript sizes at lower levels in several other tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NCAM2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu21-Gln297 of human NCAM2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
