The NAT2 isozyme functions to both activate and deactivate arylamine and hydrazine drugs and carcinogens.
Polymorphisms in this gene are responsible for the N-acetylation polymorphism in which human populations segregate into rapid, intermediate, and slow acetylator phenotypes.
Polymorphisms in NAT2 are also associated with higher incidences of cancer and drug toxicity
NAT2 encodes the polymorphic NAT protein because the gene product had an identical apparent molecular weight to that of NAT detected in human liver cytosol and the deduced amino acid sequence of NAT2 contained 6 peptide sequences that had previously been determined from tryptic peptides of the polymorphic NAT purified from human liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat NAT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Asp20-Val280 of rat NAT2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
