Nuclear autoantigenic sperm protein is a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells.
Multiple isoforms are encoded by transcript variants of this gene.
The protein sequence is 53% identical to that of the Xenopus ortholog, and 85% identical to that of the rabbit ortholog when the N terminal of the rabbit sequence is aligned with amino acid 101 of the human sequence.
Two histone-binding domains are strongly conserved.
Northern blot analysis revealed a 3.2 kb mRNA.
Immunolocalization experiments demonstrated that in the testis, NASP is found primarily in the cytoplasm of primary spermatocytes and around or within the nuclei of spermatids.
NASP is present near the acrosomal region in mature human sperm.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat NASP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Thr3-Asp223 of rat NASP fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
