NANOG is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells.
Human NANOG protein is a 305aa protein with a conserved homeodomain motif that is localized to the nuclear component of cells.
The homeodomain region facilitates DNA binding.
There are N-terminal, homeodomain, and C-terminal regions in human NANOG protein.
Like murine NANOG, N-terminal region of human NANOG is rich in Ser, Thr and Pro residues and C-terminus contains W repeats.
The homeodomain in hNANOG ranges from residues 95 to 155.
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency.
NANOG is thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry:: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NANOG.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val305 of human NANOG, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
