N-Acetyl-Beta-D-glucosaminidase (abbreviated to as NAGase) is an enzyme in lysosome which is contained abundantly in the renal tubular epithelia and is related to the degradation of mucopolysaccharides and glycoproteins
It is recognized that the NAGase in urine increases in various cases of renal diseases or after an operation of the kidney and the NAGase in urine as well as serum increases in case of diabetes.
In the field of clinical and animal experiments, the measurement of the NAGase has attracted an interest in connection with diagnosis of various renal diseases and also as an indicator in examining nephrotoxicity.
In the prior art, p-nitrophenyl N-acetyl-Beta-D-glucosaminide and 4-methylumbelliferyl-N-acetyl-Beta- D-glucosaminide have generally been used in the determination of the NAGase activity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse NAGase.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile695-Leu814 of mouse NAGase, fused to N-terminal His-Tag expressed in E. coli.
