MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines
The deduced 1,320aa protein has a calculated molecular mass of 14kD.
It contains 5 immunoglobulin (Ig) repeats and 6 interdomain insertions.
RNA dot blot analysis of multiple human tissues detected expression restricted to adult skeletal muscle and adult and fetal heart; fetal skeletal muscle was not examined.
Immunofluorescent staining revealed that rat Mypn localized at the Z line of both heart and skeletal myofibrils, and it colocalized with cardiac ankyrin repeat protein (Carp) within the I band.
Mypn localized to the nucleus in about 75% of isolated rat cardiac myocytes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MYPN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro945-Leu1320 of human MYPN, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
