(2006) demonstrated that MYO1F is expressed predominantly in the mammalian immune system.
Cells from Myo1f-deficient mice exhibited abnormally increased adhesion and reduced motility, resulting from augmented exocytosis of beta-2 integrin-containing granules.
In addition, the cortical actin that colocalizes with Myo1f was reduced in Myo1f-deficient cells.
In vivo, Myo1f-deficient mice showed increased susceptibility to infection by Listeria monocytogenes and an impaired neutrophil response.
Thus, Kim et al.
(2006) concluded that Myo1f directs immune cell motility and innate host defense against infection.
The deduced 1,099aa protein is 91% identical to the mouse protein.
Northern blot analysis revealed wide expression of Myo1f in adult mouse tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MYO1F.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to His655-Ser922 of rat MYO1F, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
