Myoglobin is a single-chain globular protein of 153aa, containing a heme prosthetic group in the center around which the remaining apoprotein folds.
It has eight alpha helices and a hydrophobic core.
It has a molecular weight of 16,700 daltons, and is the primary oxygen-carrying pigment of muscle tissues.
Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperativity is a property of multimeric/oligomeric proteins only.
Instead, the binding of oxygen by myoglobin is unaffected by the oxygen pressure in the surrounding tissue.
Myoglobin is often cited as having an "instant binding tenacity" to oxygen given its hyperbolic oxygen dissociation curve.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes sheep MYO.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Sh
Immunogen：Recombinant protein corresponding to Met1-Gly154 of sheep MYO, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
