Myosin is composed of heavy chain (MHC) and light chain (MLC) subunits.
Alkaline MLCs, such as MLC1SA, dissociate from MHCs at high pH.
The deduced 208aa protein has a calculated molecular mass of 22.7kD.
The N terminus is enriched in lysine, proline, and alanine residues, characteristic of alkaline MLCs.
Northern blot analysis of rabbit muscle mRNA detected prominent expression in soleus, a slow-twitch skeletal muscle, with lower expression in fast-twitch muscle.
In human, MLC1SA expression was detected in fibroblasts, primary myoblasts, and adult and fetal muscle.
In mouse, Mlc1sa was expressed in soleus muscle, testis, and brain.
Expression of MLC1SA appeared to be primarily associated with early and mature myotubes at the onset of muscle differentiation in both mice and humans.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MYL6B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Lys5-Glu199 of human MYL6B, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
