Myosin is a hexameric ATPase cellular motor protein.
It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains.
MYL6 is a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues.
Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene
Both deduced proteins contain 151aa and differ from other MLCs in that they lack highly basic amino aids in their N termini.
MLC3NM has a calculated molecular mass of 16.9kD, whereas MLC3SM has a calculated molecular mass of 17kD.
Expression of MYL6 was highest in fibroblasts and myoblasts, lower in fetal muscle, and lowest in adult muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MYL6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe4-Arg146 of human MYL6, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
