84-4428-72　MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult, MYHSA1,MYHa, MyHC-2X/D, Myosin-1, Myosin Heavy Chain IIx/d) 200ul　364265

特徴

• Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP.
• Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains.
• Myosin heavy chains are encoded by a multigene family.
• In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells.
• Woodhead et al.
• (2005) analyzed the structure of a phosphorylation-regulated striated muscle thick filament using cryoelectron microscopy.
• Three-dimensional reconstruction and atomic fitting studies suggest that the interacting head structure is also present in the filament, and that it may underlie the relaxed state of thick filaments in both smooth and myosin-regulated striated muscles.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YAGTVD YNIAGWLDKN KDPLNETVVG LYQKSAMKTL ALLFVGATGA EAEAGGGKKG GKKKGSSFQT VSALFRENLN KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL GSIDIDHTQY KFGHTKVFFK AGLLGLLEEM RDEKLAQLIT RTQA
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.