The MYCBP gene encodes a protein that binds to the N-terminal region of MYC and stimulates the activation of E box-dependent transcription by MYC.
The MYC protooncogene appears to play key roles in cell proliferation, differentiation, transformation, and apoptosis.
A variety of functions of the MYC gene product are attributable to protein-protein interactions with various cellular factors.
AMY1 was found to bind in vitro and in vivo to the regulatory subunit II-binding region of AKAP1 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis.
AMY1 was expressed postmeiotically in the testis, as was also S-AKAP84.
AMY1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively.
These results suggested that AMY1 plays a role in spermatogenesis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MYCBP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala2-Glu103 of human MYCBP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
