Myosin-binding protein C, slow-type is a protein that in humans is encoded by the MYBPC1 gene.Myosin-binding protein C (MYBPC) is a myosin-associated protein of unknown function found in the cross-bridge-bearing zone, or C region, of A bands in striated muscle.
Using a cDNA clone encoding the fast-type isoform of chicken MYBPC, Weber et al.
(1993) screened a human fetal muscle cDNA library and isolated clones encoding the full-length human fast-type isoform of MYBPC.
The cDNA for the fast type encodes a polypeptide of 1,142aa with an expected molecular mass of 128 kD.
They also isolated the slow-type isoform of human MYBPC (MYBPC1) and fully sequenced it.
By study of somatic cell hybrids, Weber et al.
(1993) mapped the gene for the slow type of myosin-binding protein C to human chromosome 12.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse MYBPC1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Leu277 of mouse MYBPC1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
