Mucins are high molecular mass, highly glycosylated macromolecules that are the major components of mucus secretions.
MUC5B is a salivary mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva.
It is composed of 14.9% protein, 78.1% carbohydrate, and 7% sulfate.
Desseyn et al.
isolated MUC5B genomic sequence.
They found that the central exon encodes a predicted 3,570aa peptide containing 19 subdomains that can be grouped into 4 larger composite units referred to as 'superrepeats'.
Since MUC5B and the related proteins MUC2 and MUC5AC are clustered within chromosome 11p15.5, suggesting that they are members of a multigene family.
Desseyn et al. reported that the region 3-prime of the large central exon of MUC5B is composed of 18 exons and spans about 10.7 kb.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes bovine MUC5B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo
Immunogen：Recombinant protein corresponding to Gln6489-Tyr6632 of bovine MUC5B, expressed in E. coli.
