The rodent and human MUC13 cDNAs contain a series of CA dinucleotide repeats and an alternative polyadenylation signal.
The deduced 512aa human protein has a calculated molecular mass of 54.7kD.
Both human and rodent MUC13 have an N-terminal signal peptide, followed by a serine/threonine-rich mucin repeat domain, a cysteine-rich EGF-like domain, a SEA module, 2 more cysteine-rich EGF-like domains, a transmembrane region, and a cytoplasmic tail.
The mucin repeat domain is predicted to be heavily O-glycosylated, and MUC13 also has sites for N-glycosylation and phosphorylation.
Northern blot analysis detected highest expression of a 3.2-kb transcript in colon and trachea, with moderate levels in kidney, small intestine, appendix, and stomach, and low levels in several other tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MUC13.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser174-Gln404 of human MUC13, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
