Myotrophin, a 12kD protein, was originally isolated by Sen et al.
(1990) from the hypertrophied ventricles of spontaneously hypertensive rats and later by Sil et al.
(1993) from dilated cardiomyopathic human hearts.
Mukherjee et al.
(1993) found that myotrophin increases the transcript levels of protooncogenes as well as the transcript levels of beta-myosin heavy chain and ANF, markers for cardiac hypertrophy.
The 117aa protein contained an acetylated N terminus and 2.5 internal 33aa ankyrin repeats.
Sivasubramanian et al.
(1996) identified a second half-ankyrin repeat in rat Mtpn.
Northern blot analysis detected up to 7 transcripts between 0.4 and 4.3 kb expressed in all rat tissues examined.
Highest levels were detected in brain, and lowest levels in skeletal muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA:1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse MTPN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Cys2-Gln118 of mouse MTPN, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
