Methylenetetrahydrofolate reductase (EC 1.5.1.20) catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.
Sequence data from the porcine DNA were then used to screen a human liver cDNA library by PCR for isolation of a partial human cDNA, the predicted amino acid sequence of which showed strong homology to porcine MTHFR and to bacterial metF genes.
The encoded 656aa protein is a homodimer with a total molecular mass of 150kD.
The human and mouse MTHFR proteins share approximately 90% identity.
The different sized transcripts resulted from alternate transcription start sites and polyadenylation signals
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MTHFR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Arg325 of human MTHFR, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
