Mammalian mitochondria, however, have a single Met-tRNA gene (MTTM) which must be processed to produce both formylated and nonformylated forms.
Presumably MTFMT balances the ratios of both species of Met-tRNA to match the needs of protein synthesis.
Takeuchi et al.
(1998) partially purified a mitochondrial methionyl-tRNA transformylase (MTFmt) from bovine liver, determined the amino-terminal peptide sequence, and obtained a cDNA from a bovine heart library based on a partial human cDNA sequence identified in a database search.
The predicted 357aa bovine and 304aa human proteins are about 30% identical to MTFs in prokaryotes.
The International Radiation Hybrid Mapping Consortium mapped the MTFMT gene to chromosome 15.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes mouse MTFMT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val78-Ser386 of mouse MTFM, expressed in E. coli.
