84-4428-37　MTF1 (Metal Regulatory Transcription Factor 1, MRE-binding transcription factor) 200ul　364230

特徴

• Metal regulatory transcription factor 1 is a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver.
• The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE)
• The human protein has 753 aa with 93% aa sequence identity to the mouse protein and has an extension of 78 aa at the C terminus without counterpart in the mouse.
• In the 2 species the factor has the same overall structure, including 6 zinc fingers in the DNA binding domain.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control Included:364230A: MTF1, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
• Usage: 10ul per when DAB is the substrate; 5ul per well when used in ECL
• Store at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.