Metallothionein (MT) is a family of cysteine-rich, low molecular weight (MW ranging from 3500 to 14000Da) proteins.
MTs have the capacity to bind both physiological (such as zinc, copper, selenium) and xenobiotic (such as cadmium, mercury, silver, arsenic) heavy metals through the thiol group of its cysteine residues, which represents nearly the 30% of its amino acidic residues.Karin and Richards (1982) cloned a human MT-II gene and determined the sequence of the mRNA.
It showed a GC-rich sequence; 79% of codons had G or C residues at the third position
Bates and Mulley (1988) identified a BamHI RFLP at the MT2A locus.
This locus was assigned to 16q21 by in situ hybridization to chromosomes expressing FRA16B and FRA16C, 2 fragile sites on chromosome 16.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Ala61 of rat MT2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
