84-4428-22　MSE (ENolase, Muscle Specific, ENO3, Beta Enolase, Enolase 3, 2-phospho-D-glycerate hydro-lyase, Skeletal muscle enolase) 200ul　364215

特徴

• Beta-enolase is one of the three enolase isoenzymes found in mammals.
• This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult.
• A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents.
• Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
• Two transcripts have been identified for this gene that differ only in their 5' UTR
• Feo et al.
• (1990) mapped the muscle-specific beta-enolase (ENO3) to chromosome 17pter-p11 by analysis of rodent-human somatic cell hybrids and transfectant cell lines carrying different portions of chromosome 17.
• This muscle-specific enzyme maps to the region encoding the gene cluster for the sarcomeric myosin heavy chains (MYH1).
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK AKFGANAILG VSLAVCKAGA AEKGVPLYRH IADLAGNPDL ILPVPAFNVI NGGSHAGNKL AMQEFMILPV GASSFKEAMR IGAEVYHHLK GVIKAKYGKD ATNVGDEGGF APNILENNEA LELLKTAIQA AGYPDKVVIG MDVAASEFYR NGKYDLDFKS PDDPARHITG EKLGELYKSF IKNYPVVSIE DPFDQDDWAT WTSFLSGVNI QIVGDDLTVT NPKRIAQAVE KKACNCLLLK VNQIGSVTES IQACKLAQSN GWGVMVSHRS GETEDTFIAD LVVGLCTGQI KTGAPCRSER LAKYNQLMRI EEALGDKAIF AGRKFRNPKA K
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.