Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions.
Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.
The authors used sequence information obtained from internal fragments of the protein to design degenerate oligonucleotides which were used to amplify a fragment of the PMI cDNA.
The PMI gene encodes a predicted 423aa polypeptide.
Northern blot analysis detected a single 1.8-kb PMI mRNA in all tissues tested, with the highest levels in heart, brain, and skeletal muscle.
Recombinant PMI expressed in E.
coli had very similar activity to the native enzyme.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MPI.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala2-Leu423 of human MPI, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
