ENDOGLYX1, a 500kD cell surface glycoprotein complex, and sequenced peptides derived from the component proteins.
By searching databases using the peptide sequences, they obtained a full-length cDNA encoding MMRN2, which they called ENDOGLYX1.
The deduced 949aa protein has a calculated molecular mass of 104.4kD.
It contains an N-terminal signal sequence, followed by 2 major globular domains, an emilin (EM1) domain and a C-terminal C1q -like domain, which are separated by a large central coiled-coil-rich region.
The EM1 domain contains 7 cysteines, 6 of which likely form disulfide bridges.
The coiled-coil-rich region is divided into coiled-coil subdomains interrupted by noncoiled regions, allowing structural flexibility.
An acidic region precedes the C1q region and is typically found in proteins that interact with glycosaminoglycans.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-2ug/mlWestern Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control Included:364188A: MMRN2, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes mouse MMRN2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln23-Ala188 of mouse MMRN2, expressed in E. coli.
