84-4427-93　MMP9 (Matrix Metalloproteinase 9, GELB, Gelatinase B, CLG4B, CLG4-B, 92kD Gelatinase, 92kD Type IV Collagenase) 200ul　364187

特徴

• MMP-9 (also known as gelatinase B) is secreted as a 92kD zymogen.
• Cleavage of pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82kD.
• MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain.
• Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases.
• MMP-9 is inhibited byalpha2-macroglobulin or by TIMP-1, which binds to pro-MMP-9 as well as to active MMP-9.Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells.
• Pro-MMP-9 expression is upregulated by TGF-Beta1, IL-1Beta, TGF-alpha, PDGF-AB,
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKGAAC HFPFTFEGRS YSACTTDGRS DDMLWCSTTA DYDADRQFGF CPSERLYTQD GNADGKPCVF PFTFQGRTYS ACTSDGRSDG YRWCATTANY DQDKLYGFCP TRVDATVTGG NAAGELCVFP FTFLGKEYSA CTREGRNDGH LWCATTSNFD KDKKWGFCPD
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap