84-4427-84　MMP23B (Matrix Metalloproteinase 23B, MMP22, MMP23-B, MIFR, MIFR-1, Matrix Metalloproteinase 22, Femalysin, Matrix Metalloproteinase In The Female Reproductive Tract) 200ul　364178

特徴

• The gene for MMP-22 has been identified together with the gene for MMP-21 in the Cdc2L1-2 locus at the end of the short arm of human chromosome 1, 1p36.3, which is deleted frequently in a number of tumors (Gururajan et al, 1998).
• The gene has been renamed MMP-23B and its sequence is almost identical with that of MMP-21.
• The catalytic domain of MMP-22 is related most closely to MMP-11 (stromelysin-3).
• The protein does not contain a highly conserved cysteine residue in the proenzyme domain, shown to be involved in the autocatalytic activation of many metalloproteinases.
• Multiple MMP-22 mRNAs, some of which are derived by alternative splicing, are expressed in a tissue-specific manner.
• For other entries pertaining to metalloproteinases see also the Metalloproteinase Dictionary section of this encyclopedia.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control Included:364178A: MMP23B, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap