MMP-13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis.
In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin.
MMP-13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions.MMP-13 is expressed by several metastatic tumors including breast carcinomas, chondrosarcomas, head and neck carcinomas, and in degenerative bone diseases including rheumatoid arthritis.
Wild type and mutant p53, a tumor suppressor gene, differentially regulate MMP-13 gene expression.
Members of the activator protein-1 and core-binding factor families increase MMP-13 promoter activity in normal, differentiating osteoblasts.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine MMP13.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Asn123-Glu270 of porcine MMP13, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
