(2006) found that human umbilical vein endothelial cells in which HDAC7 (HDAC7A) expression was suppressed by RNA interference failed to adhere to each other.
Microarray analysis showed that HDAC7 suppression led to dysregulation of numerous genes encoding extracellular matrix and adhesion proteins, including 6.5-fold upregulation of MMP10 and 8.6-fold downregulation of the MMP10 inhibitor, TIMP1
Chang et al.
(2006) found that HDAC7 downregulated MMP10 by associating with MEF2, an MMP10 transcriptional activator.
Hdac7 deletion in mice, which was embryonic lethal due to widespread vascular damage, resulted in dramatic upregulation of Mmp10 in the perivascular region and downregulation of Timp1 in endothelial cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MMP10.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Glu154-Thr465 of rat MMP10, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
