Midkine, a product of a retinoic acid responsive gene, is a secreted 13kD protein belonging to the family of heparin binding growth/differentiation factors.
MK shares 45% sequence identity with other member of this family called Pleiotrophin (HB-GAM)
Midkine is composed of two domains held together by disulfide linkages.
The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity.
Part of the MK activity is enhanced by dimerization of MK.
Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation.
In the adult MK expression is restricted.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala22-Asp143 of human Midkine, expressed in E. coli.
