The two variants of the Macrophage Inflammatory Protein 1, designated MIP-1alpha and MIP-1Beta, belong to the Beta or CC chemokine subfamily.
The two chemokines were originally purified from lipopolysaccharidetreated murine monocytic cell lines, hence their designation as "Macrophage Inflammatory Protein".
MIP-1alpha and MIP-1Beta are produced by stimulated leukocytes as well as other tissue cells and tumor cells.
At least seven variants of rat MIP-1Beta, corresponding to the products of independantly cloned cDNA, including Act-2, G-26, HC-21, HIMAP, H400, pAT 744 and MAD-5, have been described.
At the protein levels, mature rat MIP-1Beta has a length of 69aa and shows a homology of 70% with MIP-1alpha.
MIP-1Beta is a natural ligand for the chemokine receptors CCR3, CCR5 and CCR8.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human MIP1b.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala24-Asn92 of human MIP1b, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
