MIP-1alpha plays an important role in the development and regulation and recruitment of leukocytes.
CCL3 is produced by a variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as both resident and recruited monocytes macrophages.
CCL3 has roles in the compartmentalization and mobilization of myeloid precursor cells (MPCs).
Through the use of CCR1 knockout mice, CCL3 has been shown to mediate the mobilization of MPCs from the bone marrow, as well as having regulatory effects on MPCs and acting to stimulate mature MPCs, but CCL3 inhibits immature cells.
CCL3 has been reported to be chemotactic for both neutrophils and monocytes in vitro and in vivo in mice.
In humans and higher primates, however, predominantly monocytic cellular infiltrates will accumulate in response to direct injection of CCL3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes canine MIP1a
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to Gln20-Ala92 of canine MIP1a, expressed in E. coli
