MIP-1alpha plays an important role in the development and regulation and recruitment of leukocytes.
CCL3 is produced by a variety of cells, including lymphocytes, fibroblasts, and epithelial cells, as well as both resident and recruited monocytes macrophages.
CCL3 has roles in the compartmentalization and mobilization of myeloid precursor cells (MPCs).
Through the use of CCR1 knockout mice, CCL3 has been shown to mediate the mobilization of MPCs from the bone marrow, as well as having regulatory effects on MPCs and acting to stimulate mature MPCs, but CCL3 inhibits immature cells.
CCL3 has been reported to be chemotactic for both neutrophils and monocytes in vitro and in vivo in mice.
In humans and higher primates, however, predominantly monocytic cellular infiltrates will accumulate in response to direct injection of CCL3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Included Protein Molecular Weight Marker:168631: Unstained Protein Molecular Weight Marker, 10-70kDSuppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol.
Ready to use; no need to heat, dilute or add reducing agents before use.
Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution.
Do not boil! Load the following volumes on SDS-PAGE gel:Mini gels: 3-5ul/wellLarge gel: 7ul/wellThe loading volumes listed above are recommended for gels with thickness of 0.75-1mm.
The loading volume should be doubled for 1.5mm thick gels
Store at -20°C Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes rat MIP1a.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala24-Ala92 of rat MIP1a, expressed in E. coli.
