Growth and expansion of tumor cells including malignant melanomas are modulated by a complex network of growth factors, which regulate proliferation and cell-matrix interaction through a variety of different signal transduction pathways
Bosserhoff et al.
(1996) cloned the gene encoding MIA and described its genomic organization.
The gene encodes a 131aa prepropolypeptide with a 24-residue secretion signal peptide sequence.
The authors identified the transcription start site of the gene and studied the expression characteristics of the gene promoter in human melanoma, epithelial, and undifferentiated cells.
Bosserhoff et al.
(1996) found by Northern blot analysis that the MIA gene was expressed in all melanoma cell lines tested but was not expressed in fibroblast or epithelial cell lines.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat MIA1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala36-Phe127 of rat MIA1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
