In addition to functioning as an MHC class II chaperone, Cd74 was required for B-cell development in mice.
B cells from mice lacking Cd74 had a low response to T-independent type II antigen and could not proliferate after mice were injected with antigen.
Study of cell surface markers showed a developmental arrest that prevented immature virgin B cells from becoming mature B cells in the periphery.
This block was independent of MHC class II expression and was an intrinsic feature of B cells that correlated with the amount of Cd74.
Using immunohistochemical and confocal microscopy analyses, Schori et al.
(2007) showed that transgenic mice that weakly expressed the p41 Cd74 isoform on an MHC I-Ab genetic background recovered better from central nervous system (CNS) injuries than did wildtype mice.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse MHCDH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu64-Val205 of mouse MHCDG, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
