Microfibrillar-associated protein 5 is a 25kD microfibril-associated glycoprotein which is rich in serine and threonine residues.
It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31kD microfibril-associated glycoprotein (MFAP2).
The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs.
The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2.
MAGP2 lacks the proline-, glutamine-, and tyrosine-rich sequences and a hydrophobic carboxyl terminus, characteristic of MAGP1.
These structural differences suggest that MAGP2 has some functions that are distinct from those of MAGP1.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.